Design Concept

Oxford Nanoimaging has redesigned super-resolution instrumentation. We have removed the superfluous elements of traditional fluorescent microscopes and by doing so have created an instrument which is optimally designed to generate high quality single-molecule data. This bottom up design removes instrument complexity and delivers a cost-effective solution, eliminating all requirements for optical tables and dedicated laser laboratories. The instrument operates on a standard laboratory bench, delivering super-resolution capabilities to a broader range of scientific researchers. The instrument supports various modes of operation: single-molecule localization-based super-resolution for quantitative cellular imaging, TIRF and epifluorescence for diffraction-limited fluorescence imaging, single-molecule FRET for measuring molecular interactions in the 2-10 nm range and single particle tracking PALM in cells.

Nanoimager super-resolution

Super-resolution techniques break the 200 nm limit on image resolution imposed by the diffraction of light. The Nanoimager achieves super-resolution by single-molecule localization (dSTORM and PALM). These techniques involve localizing only subsets of fluorophores in consecutive frames with high precision (typically better than 20 nm laterally and 50 nm axially), and reconstructing an image from the positions of the localizations.

The Nanoimager offers four laser lines with powers up to 1 W and the highest available power densities of any commercial instrument, calibrated at the sample plane and reported in real time. The two emission channels enable simultaneous dual-color imaging (four color imaging is possible through interlaced lasers). It supports colocalization studies and the capture of dynamic information for different molecular species.

3D information can be captured using the method of astigmatism for super-resolution detail, and by imaging sequential sections for greater depth.

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Unrivaled stability

The Nanoimager microscope unit has a footprint of just 21 cm by 21 cm. The compact design is space-saving, but also reduces aberrations and loss of light in the optical path.

The Nanoimager geometry inherently compensates for drift. It houses specialist materials within the solid microscope body which significantly reduce thermal drift. Multiple software components further eliminate the effect of thermal drift. In addition to the anti-drift features, it has full vibration dampening which is supported by its compact design.

Together, this produces the most stable instrument on the market with no need for an optical table.

Special features

The Nanoimager boasts the largest commercially available field of view, which is evenly illuminated throughout. The large field of view facilitates high-throughput imaging of single molecules and rapid accumulation of sample statistics.

As a Class 1 laser product, the Nanoimager can be safely operated in any room or laboratory.

The advanced sample stage has exceptional positional accuracy and reproducibility.

Autofocusing allows automated data acquisition over multiple fields of view and the rapid overview feature negates the need for oculars.

Whole-body heating to 37°C supports live-cell imaging and avoids the disruptive effect of temperature gradients.

Intelligent data analysis using our custom software suite is provided for all the Nanoimager modes of operation, including super-resolution imaging, smFRET and sptPALM.

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Super-resolution Microscopy

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Super-resolution Microscopy

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Tech Spec

Imaging and Analysis

Imaging modalities

Single-molecule imaging based 3D localization microscopy

Förster resonance energy transfer (FRET) spectroscopy

Single-molecule tracking

Achievable resolution

Lateral: exceeding 20 nm

Axial: exceeding 50 nm

Simultaneous imaging channels

2 (< 10 nm channel mapping accuracy)

Total number of imaging colors

Up to 4 lasers

Field of view

50 μm x 80 μm per channel

Software features

Real-time 3D localization analysis and rendering (sCMOS optimized)

Real-time FRET trace analysis

Clustering and co-localization analysis

Residual drift correction

Scripting interface and OMERO compatibility

Acquisition speed

100 fps full frame

5 kHz with frame height cropped to 2%

3D imaging technique

Astigmatism

Time for super-resolution full frame

Seconds to minutes (number of localizations and laser power dependent)

Operational

Focus system

One-shot autofocus

Continuous autofocus

Mechanical stability

<1 μm/K drift

<1 nm vibration amplitude (1 Hz to 500 Hz)

Illumination modes

Closed-loop, continuous illumination angle adjustment between epi-illumination and total internal reflection

Closed-loop adjustments of laser power density at sample plane

Temperature control

Resistive heating, whole instrument (for live cell imaging)

Environmental conditions

Sensor array (temperature, humidity, acceleration)

Hardware

Dimensions W x D x H

Microscope: 21 cm x 21 cm x 15 cm

Light engine: 21 cm x 42 cm x 45 cm

Camera

Latest generation sCMOS

82 % peak QE

1.6 electrons rms read noise at standard scan

Objective

Oil immersion, NA = 1.4 to NA = 1.49

Laser options

Violet: 405 nm (150 mW)

Blue: 473 nm (300 or 1000 mW), 488 nm (200 mW)

Green: 532 nm (300 or 1000 mW), 561 nm (200 or 300 mW)

Red: 640 nm (300 or 1000 mW)

Near infrared: 730 nm (300 or 1000 mW)

Laser types

DPSS and diode

Other light sources

LED for bright-field imaging

NIR auto-focus laser

Sample stage

20/20/10 mm XYZ travel range, closed-loop piezo stage with 1 nm encoder resolution

PC requirements

PC or laptop included (32GB RAM, nVidia GeForce GTX 1080/m)

Nanoimager software included, along with all future updates